人膀胱上皮永生化細胞

簡要描述：CRL-9520 SV-HUC-1 人膀胱上皮永生化細胞，
原代細胞|細胞系|細胞株|菌種；細胞庫管理規(guī)范，
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產(chǎn)品型號：SV-HUC-1
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更新時間：2024-11-12
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CRL-9520 SV-HUC-1 人膀胱上皮永生化細胞

ATCC® Number: CRL-9520™ Price: $417.00

Designations: SV-HUC-1

Depositors: Wisconsin Alumni Res. Fndn.

Biosafety Level: 2 [CELLS CONTAIN ADENOVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens （human）

Morphology: epithelial

CRL-9520 SV-HUC-1 人膀胱上皮永生化細胞

Source: Organ: ureter

Tissue: uroepithelium

Cell Type: epithelial SV40 immortalizedSV40 transformed

Cellular Products: uroepithelial keratins; SV40 T antigen

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: No

Cytogenetic Analysis: The chromosome number distribution is bimodal with 50% of the cells near diploid （mode = 44 chromosomes） and 50% tetraploid （mode = 88）. Both populations consistently showed seven marker chromosomes: 5p+, del（6）（p11）, 9q+, 11p+, 15q-, 19p+ and Xp+. Most cells lacked a normal chromosome 15. Cells in the tetraploid population did not exactly duplicate the diploid population in that most had a 14q/21 translocation involving two chromosomes 14 and two chromosomes 21, while the diploid cells had normal chromosomes 14 and 21 and a 6q/14q translocation.

Age: 11 years adult

Gender: male

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, add fresh 0.25% Trypsin, 0.03% EDTA solution and allow the flask to sit at room temperature for 20 minutes. Add fresh medium, aspirate and dispense into new flasks.

Subc*tion Ratio: 1:2 to 1:5

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2004

recommended serum:ATCC 30-2020

derivative:ATCC CRL-9519

References: 3992: Reznikoff CA, Christian BJ. Human uroepithelial cell. US Patent 4,980,290 dated Dec 25 1990

CRL-9520 SV-HUC-1

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人膀胱上皮永生化細胞

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